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KMID : 0191119920070020110
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Journal of Korean Medical Science
1992 Volume.7 No. 2 p.110 ~ p.115
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Point mutation at codon 12 of the c-Ha-ras gene in human gastric cancers
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Koh EH
Chung HC/Lee KB/Han EK/Oh SH/Min JS/Choi EM/Youn JK/Kim BS
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Abstract
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The molecular mechanisms of the carcinogenic process of gastric cancer have not been fully understood yet. In order to know whether c-Ha-ras gene is being involved in the process of gastric carcinogenesis, 8 gastric cancer cell lines, 8 cases of gastric cancer and same number of adjacent dysplasia were analyzed for the presence of mutation at codon 12, 13 and 61 of the c-Ha-ras gene by using polymerase chain reaction (PCR) and mutant-specific oligonucleotide hybridization. Point mutations at codon 12 of the c-Ha-ras gene were found in 2 out of 8 gastric cancer and dysplasia samples in one case, but we found no mutation at codon 13 or 61 of the c-Ha-ras gene. These results suggest that the frequency of mutation of the c-Ha-ras gene detected by sensitive PCR technique is low indeed, however it would be notable that such a genetic change has been detected in the dysplastic lesion of the gastric cancer patient.
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KEYWORD
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